diff --git a/test/test_utils.py b/test/test_utils.py
index 86045e680..e4e8d3825 100644
--- a/test/test_utils.py
+++ b/test/test_utils.py
@@ -667,6 +667,7 @@ ffmpeg version 2.4.4 Copyright (c) 2000-2014 the FFmpeg ...'''), '2.4.4')
         self.assertEqual(parse_dfxp_time_expr('0.1s'), 0.1)
         self.assertEqual(parse_dfxp_time_expr('00:00:01'), 1.0)
         self.assertEqual(parse_dfxp_time_expr('00:00:01.100'), 1.1)
+        self.assertEqual(parse_dfxp_time_expr('00:00:01:100'), 1.1)
 
     def test_dfxp2srt(self):
         dfxp_data = '''<?xml version="1.0" encoding="UTF-8"?>
diff --git a/youtube_dl/utils.py b/youtube_dl/utils.py
index ee20c3d9b..5b396ede8 100644
--- a/youtube_dl/utils.py
+++ b/youtube_dl/utils.py
@@ -1982,9 +1982,9 @@ def parse_dfxp_time_expr(time_expr):
     if mobj:
         return float(mobj.group('time_offset'))
 
-    mobj = re.match(r'^(\d+):(\d\d):(\d\d(?:\.\d+)?)$', time_expr)
+    mobj = re.match(r'^(\d+):(\d\d):(\d\d(?:(?:\.|:)\d+)?)$', time_expr)
     if mobj:
-        return 3600 * int(mobj.group(1)) + 60 * int(mobj.group(2)) + float(mobj.group(3))
+        return 3600 * int(mobj.group(1)) + 60 * int(mobj.group(2)) + float(mobj.group(3).replace(':', '.'))
 
 
 def srt_subtitles_timecode(seconds):